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目的:探讨姜黄素(curcumin)对高原缺氧大鼠认知功能障碍的预防作用。方法:将30只成年雄性SD大鼠随机分为健康对照组、模型组(Model组)、姜黄素[按体重60mg/(kg.d)]治疗组(curcumin组)。采用Morris水迷宫实验方法检测缺氧后大鼠学习记忆功能变化,同时检测脑片水平的LTP变化。结果:(1)模型组大鼠寻找平台的潜伏期明显长于对照组,具有统计学意义(P<0.05),姜黄素组寻找平台的潜伏期相对于模型组显著缩短(P<0.05)。撤离平台后,模型组大鼠在平台所在象限的停留时间明显短于对照组(P<0.05),姜黄素干预后大鼠在平台所在象限的停留时间较模型组显著延长(P<0.05)。(2)给予HFS刺激后各组兴奋性突出后电位(fEPSP)斜率较前明显增加,均可持续1h以上,与模型组比较缺氧组HFS刺激后fEPSP斜率明显减小(P<0.05),姜黄素可减轻缺氧所致的fEPSP斜率减小(P<0.05)。结论:姜黄素可改善慢性缺氧大鼠认知功能障碍,但其具体机制尚有待于进一步研究。
Objective: To investigate the preventive effect of curcumin on cognitive dysfunction in rats with plateau hypoxia. Methods: Thirty adult male Sprague-Dawley rats were randomly divided into healthy control group, Model group, curcumin group (curcumin group) [body weight 60mg / (kg · d)]. Morris water maze test was used to detect the changes of learning and memory function of rats after hypoxia, and to detect the changes of LTP in brain slices at the same time. Results: (1) The latent period of looking for platform in model group was significantly longer than that in control group (P <0.05). The incubation period in curcumin group was shorter than that in model group (P <0.05). After evacuation of the platform, the dwell time of rats in the model group was significantly shorter than that of the control group (P <0.05). After the curcumin intervention, the residence time of rats in the platform quadrant was significantly longer than that of the model group (P <0.05). (2) Compared with the model group, the slope of fEPSP increased significantly after HFS stimulation (P <0.05), and the slope of fEPSP significantly increased after HFS stimulation (P <0.05) Curcumin reduced the slope of fEPSP induced by hypoxia (P <0.05). Conclusion: Curcumin can ameliorate cognitive impairment in chronic hypoxic rats, but the specific mechanism remains to be further studied.