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目的：分析10%福尔马林、纯丙酮、95%乙醇、甲醇4种固定液对脑组织冷冻切片H-E染色质量的影响，筛选出一种脑组织冷冻切片的最佳固定液。方法收集手术室送检新鲜脑组织标本，经常规冷冻切片后放入不同固定液，进行H-E染色，观察脑组织冷冻切片细胞收缩程度和染色质量。结果甲醇组细胞收缩不明显，相对于其他组比较差异有统计学意义（P＜0.05）；甲醇组组织结构清晰，核浆染色鲜艳，对比度好，相对于其他组差异性显著（P＜0.001）。结论甲醇固定脑组织染色效果好，使用方便，满足术中冷冻切片的快速、准确诊断，是脑组织冷冻切片的理想固定液。“,”Objective:To screen the best fixative on brain tissue frozen section,we analysed that 4 fixatives including 10% formalin,pure acetone,95% ethanol,and methanol impact on the quality of H&E staining quality.Methods:Fresh brain tissue specimens were colected,and we made frozen sections in the different fixatives,and observed brain tissue shrinkage and dying quality with H&E staining. Results:Cel shrinkage of methanol group was significantly less than other groups (P<0.05); For methanol group,organization structure is more clear,the color is more bright and nuclear plasma contrast is higher,relative to other groups (P<0.001).Conclusion: methanol fixation method is effective on staining,easy to use,and contributing to the rapid and accurate diagnosis of intraoperative frozen section. Methanol is an ideal fixative for brain tissue frozen section.