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目的探讨乙肝HBe Ag、前S1-抗原和HBV-DNA的相关性,为临床诊疗提供实验室依据。方法乙肝HBe Ag和前S1-抗原检测采用ELISA法,HBVDNA载量采用PCR-荧光探针法。结果 (1)前S1-抗原阳性率HBe Ag(+)组91.25%(73/80)显著高于HBe Ag(-)组25.48%(40/157)(χ2=37.552,P<0.005);HBVDNA阳性率HBe Ag(+)组88.75%(71/80)亦显著高于HBe Ag(-)组29.30%(46/157)(χ2=30.508,P<0.05)。(2)HBe Ag阳性率HBV-DNA高水平复制组(即>107拷贝/ml)82.22%(37/42)显著高于低水平复制组(即103~105拷贝/ml)45.0%(9/20)(χ2=10.78,P<0.05);前S1-抗原阳性率HBV-DNA高水平复制(即>107拷贝/ml)组96.15%(42/45)亦显著高于低水平复制(即103~105拷贝/ml)组45.0%(9/45)(χ2=30.508,P<0.005)。结论乙肝HBe Ag、前S1-抗原与HBV-DNA检测,一致性较好,两者均可作为乙肝病毒复制和传染的实验室指标。
Objective To investigate the correlation between hepatitis B virus (HBeAg), pre-S1-antigen and HBV-DNA and to provide a laboratory basis for clinical diagnosis and treatment. Methods HBsAg and pre-S1-antigen of hepatitis B were detected by ELISA and HBVDNA load by PCR-fluorescent probe. Results (1) The positive rate of pre-S1-antigen was 91.25% (73/80) in HBe Ag (+) group and 25.48% (40/157) in HBe Ag (-) group (χ2 = 37.552, P <0.005) The positive rate of HBeAg (+) group was also significantly higher than that of HBe Ag (+) group (88.75%, 71/80), which was significantly higher than that of HBeAg group (29.30%, 46/157) (χ2 = 30.508, P <0.05). (2) HBe Ag positive rate82.22% (37/42) of HBV-DNA high-level replication group (> 107 copies / ml) was significantly higher than 45.0% (9/93) of low level replication group 20) (χ2 = 10.78, P <0.05). The positive rate of pre-S1-antigen was significantly higher in 96.15% (42/45) than in low-level replication ~ 105 copies / ml) group, 45.0% (9/45) (χ2 = 30.508, P <0.005). Conclusions HBeAg, pre-S1-antigen and HBV-DNA of hepatitis B have good agreement, both of which can be used as laboratory markers of hepatitis B virus replication and infection.