论文部分内容阅读
目的观察亚硒酸钠对体外培养的人胃癌BGC823细胞增殖的抑制作用及其作用机制。方法用不同浓度(0、4.0、8.0、10.0μmol/L)的亚硒酸钠处理BGC823细胞24h后,采用四甲基偶氮噻唑蓝比色法(MTT法)检测亚硒酸钠对细胞的增殖抑制作用,流式细胞仪PI染色检测细胞周期,AnnexinV/PI双染法定量检测BGC823细胞凋亡率,FAS蛋白染色流式细胞仪定量检测各实验组细胞间FAS蛋白表达,荧光显微镜观察DAPI染色细胞形态。结果亚硒酸钠可明显抑制BGC823胃癌细胞的增殖,呈剂量依赖性;亚硒酸钠可阻滞细胞于S期和增加BGC823胃癌细胞凋亡率并随剂量增大作用增强;DAPI染色观察到亚硒酸钠给药组出现细胞核浓缩、边缘化以及凋亡小体等细胞凋亡的形态特征;亚硒酸钠可呈剂量依赖地增强FAS蛋白表达。结论亚硒酸钠可显著抑制人BGC823胃癌细胞的增殖,使细胞阻滞于S期,并可诱导细胞凋亡,其机制可能与上调FAS蛋白有关。
Objective To observe the inhibitory effect of sodium selenite on the proliferation of human gastric cancer cell line BGC823 and its mechanism. Methods BGC823 cells were treated with different concentrations of sodium selenite (0, 4.0, 8.0 and 10.0 μmol / L) for 24 h, and the effects of sodium selenite on the proliferation of cells were evaluated by MTT assay Proliferation inhibition, flow cytometry PI staining to detect cell cycle, AnnexinV / PI double staining quantitative detection of apoptosis rate BGC823 cells, FAS protein staining flow cytometry quantitative detection of intracellular FAS protein expression in each experimental group, the fluorescence microscope DAPI Stained cell morphology. Results Sodium selenite could significantly inhibit the proliferation of BGC823 gastric cancer cells in a dose-dependent manner. Sodium selenite could block cells in S phase and increase the apoptosis rate of BGC823 gastric cancer cells with increasing doses. DAPI staining Sodium selenite administration group showed morphological features of cell apoptosis such as nuclear condensation, marginalization and apoptotic bodies. Sodium selenite enhanced the expression of FAS protein in a dose-dependent manner. Conclusion Sodium selenite can significantly inhibit the proliferation of human BGC823 gastric cancer cells, arrest the cells in S phase and induce apoptosis, which may be related to the up-regulation of FAS protein.