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目的：研究大黄对烫伤后大鼠肠粘膜上皮细胞线粒体呼吸链的影响。方法：采用大鼠背部２５％ Ⅲ度烫伤模型，分离小肠粘膜上皮细胞内线粒体，监测细胞色素ａａ３ 、ｂ、ｃ和ｃ１ 的水平，并观察大黄在其中的影响。结果：烫伤后２ 小时肠粘膜上皮细胞线粒体细胞色素ａａ３、ｂ 和ｃ１ 较为稳定，而在伤后６小时出现明显丢失，与烫伤组０．５ 和２ 小时及对照组比较，Ｐ均＜ ０．０５，而细胞色素ｃ在烫伤后早期即有明显丢失，于伤后６ 小时最为严重，与对照组比较Ｐ＜ ０．０５；大黄治疗后细胞色素ａａ３、ｂ、ｃ和ｃ１ 丢失显著减少，与烫伤组比较Ｐ均＜ ０．０５。结论：烫伤能导致肠粘膜上皮细胞线粒体呼吸链明显受损，而大黄对其有很好的保护作用。
Objective: To study the effect of rhubarb on the mitochondrial respiratory chain of intestinal mucosal epithelium in burned rats. METHODS: Using a 25% III degree scald model on the rat’s back, the mitochondria in the intestinal mucosal epithelial cells were isolated and the levels of cytochrome aa3, b, c, and c1 were monitored, and the effects of rhubarb on them were observed. RESULTS: The mitochondrial cytochrome aa3, b, and c1 of the intestinal mucosal epithelium cells were stable at 2 hours after scald, and they were significantly lost at 6 hours after injury. Compared with the scald group at 0.5 and 2 hours and the control group, P<0. 05, while cytochrome c was significantly lost early in the scald, most severe at 6 hours after injury, compared with the control group P<0.05; cytochrome aa3, b, c, and c1 loss after rhubarb treatment was significantly reduced, and The burn group compared P<0.05. Conclusion: Scald can lead to significant impairment of the mitochondrial respiratory chain of intestinal epithelial cells, while rhubarb has a good protective effect.