论文部分内容阅读
21只SD大鼠,用氨基甲酸乙酯麻醉(1g/kg,ip),以膈肌放电和气管内压作为呼吸的指标。向双侧面神经后核内侧区(mNRF)注入0.25~0.5mol/Lγ-氨基丁酸(GABA)0.5μl后,动物出现明显的呼吸抑制,呼吸频率减慢,膈肌电活动减少。予先使用GABA受体阻断剂荷包牡丹硷(bicuculline)可拮抗或翻转GABA的呼吸抑制效应。结果提示,GABA是中枢呼吸调节的重要神经介质,其作用可能是通过作用于mNRF的呼吸神经元实现的。
Twenty-one SD rats were anesthetized with urethane (1 g / kg, ip) and diaphragmatic discharge and endotracheal pressure were taken as indices of respiration. After injecting 0.5μl of 0.25 ~ 0.5mol / L γ-aminobutyric acid (GABA) into the medial area of the posterior nucleus of the bilateral facial nerve (mNRF), the animals showed obvious respiratory depression, decreased respiratory rate and decreased diaphragmatic electrical activity. Pretreatment with GABA receptor antagonist bicuculline antagonizes or reverses the respiratory inhibitory effect of GABA. The results suggest that GABA is an important neurotransmitter of central respiratory regulation, and its effect may be through the respiratory neurons acting on mNRF.