血清总巯基测定目前多采用比色法,此法虽经多次改进,但灵敏度尚低,且使用了剧毒物质氰化钾。 示波极谱测定巯基未见报道,但电化学已广泛应用于地质、医药和环境等领域。能否用于生化指标测定?经研究发现:巯基硝化、重氮化后与萘乙二胺络合所形成的络合物,于波峰电位-0.5伏处有一灵敏波。进一步探讨实验条件得知:在盐酸介质中硝化巯基及选择适当的硝化,偶氮酸度,氯化汞和磺胺用量,能大大提高灵敏度。 Determination of total thiol serum colorimetric method, although this method has been repeatedly improved, but the sensitivity is still low, and the use of highly toxic substances potassium cyanide. Oscillographic polarographic determination of sulfhydryl has not been reported, but electrochemical has been widely used in geology, medicine and the environment. Can be used for biochemical determination? The study found: mercapto-nitration, after diazotization with naphthylethylenediamine complex complex formed at the peak potential of -0.5 volts at a sensitive wave. To further explore the experimental conditions that nitration of thiol in hydrochloric acid medium and the appropriate choice of nitration, azo acidity, mercuric chloride and sulfonamide dosage, can greatly improve the sensitivity.