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目的观察尼索地平(nifedipine,NIS)对KCl和去氧肾上腺素(phenylephrine,PHE)所致离体大鼠肺动脉收缩的作用,同时研究其对野百合碱诱导的肺动脉高压(pulmonary hypertention,PH)大鼠的影响。方法 1采用离体血管实验法,以KCl和PHE在含钙离子和不含钙离子的K-H液中预收缩离体大鼠肺动脉环,观察NIS对血管收缩的影响。2取40只Wistar大鼠随机分为正常组、模型组、NIS组和硝苯地平(NIF)组,每组10只,采用野百合碱(50 mg·kg?1)皮下注射建立大鼠PH模型,连续灌胃给药21 d。采用右心导管法测定m PAP、m RVP、max PAP和max RVP,并计算各组大鼠的RVHI和LI。结果 1在含钙离子的K-H液中,NIS抑制KCl(20,40,60 mmol·L?1)和PHE(1×10?7,5×10?7,1×10?6 mol·L?1)所致血管收缩的IC50分别为0.87×10?8,1.41×10?8,8.32×10?8 mol·L?1和0.56×10?7,4.27×10?7,1.28×10?6 mol·L?1。在无钙离子K-H液中,NIS无抑制离体血管收缩的作用。2模型组大鼠m PAP、m RVP、max PAP、max RVP、RVHI和LI均显著高于正常组(P<0.01),NIS组和NIF组大鼠的上述指标均显著低于模型组(P<0.05)。结论 NIS有抑制大鼠肺动脉收缩的作用,对野百合碱诱导的大鼠PH有治疗作用。
Objective To observe the effect of nifedipine (NIS) on pulmonary artery contraction induced by KCl and phenylephrine (PHE) in rats, and to study the effects of nifedipine on pulmonary hypertention (PH) Effects of rats. Method 1 The isolated rat pulmonary artery rings were precontracted with KCl and PHE in K-H solution containing calcium ions and calcium ions, and the effect of NIS on vasoconstriction was observed. Forty Wistar rats were randomly divided into normal group, model group, NIS group and nifedipine (NIF) group, 10 rats in each group. Rats were subcutaneously injected with 50 mg · kg -1 monocrotaline Model, continuous intragastric administration of 21 d. Right heart catheterization was used to determine mPAP, m RVP, max PAP and max RVP, RVHI and LI were calculated. Results 1 In KH solution containing calcium ions, NIS inhibited KCl (20, 40, 60 mmol·L -1) and PHE (1 × 10 -7, 5 × 10 -7, 1 × 10 -6 mol·L -1) 1) induced vasoconstriction were 0.87 × 10 8, 1.41 × 10 8, 8.32 × 10 8 mol·L -1 and 0.56 × 10 7, 4.27 × 10 7, 1.28 × 10 6 respectively mol·L -1. In calcium-free K-H solution, NIS did not inhibit ex vivo vasoconstriction. 2 in model group were significantly higher than those in normal group (P <0.01), while those in NIS group and NIF group were significantly lower than those in model group (P <0.05). Conclusion NIS can inhibit the contraction of rat pulmonary artery and has a therapeutic effect on the PH induced by monocrotaline in rats.