论文部分内容阅读
作者等1997年8-10月,参照Rieckmann体外微量测定法原理,制作了蒿甲醚测定板,并在云南南部测定了恶性疟原虫对该药及咯萘啶、氯喹的敏感性。测定成功率分别为76.19%(32/42)、72.50%(29/40)和73.81%(31/42);ID50依次为180、72及128nmol/L;ID95为1600、416及640nmol/L。蒿甲醚板与氯喹板相比,测定成功率相同,疟原虫在各浓度药井中的抑制率亦同氯喹板一样,随药物浓度的增高而依次上升,而裂殖体形成例数依次减少。提示本文制作的蒿甲醚测定板药性稳定,测定结果可靠,可用于现场监测。咯萘啶与以往测定结果相比,敏感性又有所下降。
The author from August to October 1997, with reference to Rieckmann in vitro microassay principle, made artemether determination plate, and in southern Yunnan measured the Plasmodium falciparum and the drug pyronaridine, chloroquine sensitivity. The success rates were 76.19% (32/42), 72.50% (29/40) and 73.81% (31/42), respectively. ID50 was 180, 72 and 128 nmol / L, ID95 was 1600, 416 and 640 nmol / L. Artemether plate and chloroquine plate compared to the same success rate, the inhibition rate of Plasmodium in the same concentration of drug wells with chloroquine board, with the increase of drug concentration in turn increased, while the number of schizonts formed in order to reduce. Tip artemether assay plate made in this paper stable, reliable determination results can be used for on-site monitoring. Pyronaridine compared with the previous test results, the sensitivity has declined.