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[目的]观察冰茶栓含药血清对体外培养的胃癌SGC-7901细胞凋亡的诱导作用。[方法]通过血清药理学的方法制备冰茶栓含药血清。将冰茶栓含药血清与体外培养的人胃癌SGC-7901细胞共同培养48h后,应用光学显微镜观察细胞形态的变化;用DNA琼脂糖凝胶电泳检测DNA片段的梯状条带;用流式细胞术检测凋亡峰(亚G1峰)来分析研究冰茶栓含药血清诱导的细胞凋亡。[结果]经过冰茶栓含药血清作用后,SGC-7901细胞在显微镜下呈现典型的凋亡形态学改变;DNA琼脂糖凝胶电泳有特异性梯状条带出现;流式细胞仪检测出现凋亡峰,并显示凋亡率为27.44%,而空白血清组凋亡率仅为4.71%。[结论]冰茶栓含药血清可诱导胃癌细胞SGC-7901发生凋亡,为冰茶栓临床抗胃癌治疗提供实验依据。
[Objective] To observe the induction effect of Bingcha Suppositories on apoptosis of gastric cancer SGC-7901 cultured in vitro. [Method] The serum containing Xuebian suppository was prepared by serum pharmacology. After incubated with serum of Bingtong suppository for 48h in vitro and cultured in vitro, the morphological changes of cells were observed under light microscope. The DNA ladder was detected by DNA agarose gel electrophoresis. Apoptotic peak (sub-G1 peak) was detected by cytometry to analyze the apoptosis induced by serum containing iced tea suppositories. [Result] The SGC-7901 cells showed the typical morphological changes of apoptosis under the microscope after the serum containing Bingcha suppository was treated. The specific ladder-like bands appeared on the DNA agarose gel electrophoresis. The results of flow cytometry Apoptotic peak, and showed that the apoptosis rate was 27.44%, while the blank serum group apoptosis rate was only 4.71%. [Conclusion] Bingtong suppository serum can induce the apoptosis of gastric cancer cell SGC-7901 and provide experimental evidence for its clinical anti-gastric cancer treatment.