论文部分内容阅读
目的 探讨脂蛋白脂酶(LPL)基因中常见多态位点HindⅢ基因多态性与儿童单纯肥胖之间的关系。方法 用聚合酶链反应(PCR)和限制性内切酶片段长度多态性(RFLP)技术,检测92 例单纯肥胖儿童和80 例正常儿童的HindⅢ-LPL基因多态性,测定血脂、脂蛋白含量及体块指数(BMI)、血压、皮褶厚度等。结果 肥胖组H+ H+ -LPL基因型频率及H+ 等位基因频率分别为59.78% 和79.35% ,明显高于正常对照组。肥胖组H+ H+ -LPL基因型亚组的TG、TC、LDL-C、ApoB含量以及BMI、舒张压、肱二头肌皮褶厚度、肩胛下皮褶厚度等,均明显高于H+ H- -LPL基因型亚组。结论 H+ H+ 与儿童单纯肥胖相关,等位基因H+ 可能是儿童单纯肥胖的一种遗传易感标志
Objective To investigate the association between polymorphism of Hind Ⅲ gene in common lipoprotein lipase (LPL) gene and simple obesity in children. Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to detect HindⅢ-LPL gene polymorphism in 92 cases of simple obesity children and 80 normal children. The levels of lipids, lipoproteins Content and body mass index (BMI), blood pressure, skinfold thickness and so on. Results The frequencies of H + H + -LPL genotype and H + allele in obesity group were 59.78% and 79.35%, respectively, which were significantly higher than those in normal control group. The contents of TG, TC, LDL-C, ApoB, BMI, diastolic blood pressure, biceps skinfold thickness and subscapular skinfold thickness in H + H + -LPL genotype subgroup were significantly higher than those in H + H- LPL genotype subgroup. Conclusions H + H + is associated with simple obesity in children, allele H + may be a genetic predisposition to childhood obesity