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目的比较脂氧素A4受体(lipoxin A4 receptor,ALX)在人白血病细胞K562与HL60细胞的表达情况,研究脂氧素A4(Lipoxin A4,LXA4)对K562与HL60膜受体ALX的表达影响,探索LXA4对K562与HL60细胞作用效应强弱不同的可能因素。方法体外培养人白血病细胞K562与HL60,实验分为空白组和LXA4(50、100、200 nmol/L)处理组。采用免疫荧光技术检测K562和HL60细胞是否表达LXA4受体ALX;用不同浓度LXA4作用24 h后,RT-PCR和Western blotting检测并比较各实验组K562与HL60膜受体ALX的表达变化。结果免疫荧光检测显示,K562与HL60胞膜周围及胞浆内有明显绿色荧光,提示二者表达LXA4受体ALX;RT-PCR和Western blotting检测结果表明:随LXA4作用浓度的增高,K562与HL60细胞膜受体ALX的表达呈增高趋势(P<0.05),组间比较,ALX表达差异无统计学意义(P>0.05);K562与HL60空白组比较即从基础水平比较ALX的表达水平无明显差异(P>0.05)。结论 LXA4可上调K562与HL60膜受体ALX的表达,且LXA4对K562与HL60效应强弱的不同并非ALX基础表达水平不同所导致,可能存在其他影响因素。
Objective To compare the expression of lipoxin A4 receptor (ALX) in human leukemia K562 and HL60 cells and the effect of Lipoxin A4 (LXA4) on the expression of ALX in K562 and HL60, To explore the LXA4 K562 and HL60 cell effect on the strength of the different possible factors. Methods Human leukemia cells K562 and HL60 were cultured in vitro. The experiment was divided into blank group and LXA4 (50,100,200 nmol / L) treatment group. Immunofluorescence was used to detect the expression of LXA4 receptor ALX in K562 and HL60 cells. After treated with different concentrations of LXA4 for 24 h, the expression of ALX in K562 and HL60 was detected by RT-PCR and Western blotting. Results The results of immunofluorescence showed that there was obvious green fluorescence around the cytoplasm of K562 and HL60 cells, suggesting that LXA4 receptor ALX was expressed by both of them. The results of RT-PCR and Western blotting showed that with the increase of LXA4 concentration, K562 and HL60 (P <0.05). There was no significant difference in the expression of ALX between the two groups (P> 0.05). There was no significant difference in ALX expression between K562 and HL60 blank groups (P> 0.05). Conclusions LXA4 up-regulates the expression of ALX in K562 and HL60, and the effect of LXA4 on K562 and HL60 is not caused by the different basic expression of ALX. Other influencing factors may exist.