论文部分内容阅读
应用 Epstein- Barr病毒编码的小 RNA- 1(Epstein- Barr virus encoded sm all RNA- 1,EBER- 1)分子探针进行原位分子杂交 ,检测沈阳地区鼻咽癌的 Epstein- Barr病毒存在状况及意义。结果发现 :EBER- 1杂交信号定位于鼻咽癌的癌细胞核 ,在正常的鼻咽粘膜上皮细胞、间质细胞、粘液腺及淋巴细胞均为阴性。在 5 3例鼻咽癌病例中 ,37例阳性 ,阳性率为 6 9.8%。EBER- 1的阳性表达与鼻咽癌的分化程度呈负相关 ,与患者的生存期未见明显关系。提示 :应用 EBER- 1分子探针可进行回顾性研究 ;EB病毒的存在与鼻咽癌的分化程度成负相关。
The Epstein-Barr virus encoded Epstein-Barr virus (EBER-1) molecular probe was used to detect the presence of Epstein-Barr virus in nasopharyngeal carcinoma in Shenyang region by in situ hybridization. significance. The results showed that EBER-1 hybridization signal localized in the nucleus of NPC cells and negative in normal nasopharyngeal epithelial cells, interstitial cells, mucous glands and lymphocytes. Of 53 cases of NPC, 37 were positive and the positive rate was 6.8%. The positive expression of EBER-1 was negatively correlated with the differentiation of nasopharyngeal carcinoma and had no significant relationship with the survival of patients. Tip: The use of EBER-1 molecular probe can be retrospectively studied; the presence of Epstein-Barr virus and nasopharyngeal carcinoma is a negative correlation between the degree of differentiation.