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小鼠脾细胞体外培养３ｈ即可检测到ＩＬ１αｍＲＮＡ的表达，４ｈ达到高峰，５ｈ后降至不能检出的水平。ＴＮＦαｍＲＮＡ在４ｈ开始出现，４．５ｈ时达到高峰，５．５ｈ后逐渐消失。在培养开始时分别加入灵芝多糖ＧＬＢ７５、１０、２０、４０μｇ／ｍｌ，４ｈ及４．５ｈ分别检测ＩＬ１α及ＩＬ１αｍＲＮＡ的表达情况。结果ＧＬＢ７能促进ＩＬ１α及ＩＬ１αｍＲＮＡ的表达，对ＩＬ１αｍＲＮＡ的表达提高率分别为７．６％、６７．３％、１０５．０％、１４３．１％；对ＴＮＦαｍＲＮＡ的表达提高率分别为９．９％、３３．９％、４５．９％、７５．８％。８ｈ后ＧＬＢ７对培养上清中ＩＬ１活性的提高率分别为１．４％、３．０％、６．７％、８．１％；１２ｈ后对培养上清中ＴＮＦα活性的提高率分别为３．０％、１４．１％、２８．２％、３５．７％。结果说明灵芝多糖ＧＬＢ７能通过促进小鼠脾细胞原代培养时ＩＬ１α及ＴＮＦαｍＲＮＡ的表达从而促进ＩＬ１及ＴＮＦα的合成与分泌。
Mouse spleen cells cultured in vitro 3h to detect IL 1α mRNA expression, 4h peak, 5h after the level can not be detected. TNFα mRNA began to appear at 4h, peaked at 4.5h and gradually disappeared after 5.5h. At the beginning of culture, Ganoderma lucidum polysaccharide GLB75, 10, 20, 40μg / ml, 4h and 4.5h were added respectively to detect the expression of IL1α and IL1αmRNA. Results GLB7 can promote the expression of IL1α and IL1αmRNA, and the increase rate of IL1αmRNA expression was 7.6%, 67.3%, 105.0% and 143.1% respectively; the increase rate of TNFαmRNA expression Respectively, 9.9%, 33.9%, 45.9%, 75.8%. 8 h after GLB7 in the culture supernatant IL 1 activity were 1.4%, 3.0%, 6.7%, 8.1%; 12h after the culture supernatant TNFα activity were increased 3.0%, 14.1%, 28.2%, 35.7%. The results showed that GLB7 can promote the synthesis and secretion of IL1 and TNFα by promoting the expression of IL1α and TNFα mRNA in the primary culture of mouse spleen cells.