细胞质型多角体病毒的多角体,用普通染色法较难着色,特别是组织切片,用碱性染料,如吡囉红(Phronine)、硷性天蓝(thionine blue)、甲苯胺蓝(toluidine blue)等也只能染上很淡的颜色。近年来,我们在检查马尾松毛虫质型多角体病毒时同样碰到这种困难。后来通过各种染料的比较染色试验,经反复实践,找到了简单易行的染色方法,现介绍如下。 Polyhedra of cytoplasmic polyhedrosis viruses are harder to stain using conventional staining methods, particularly tissue sections, using basic dyes such as Phronine, thionine blue, toluidine blue, And so can only be dyed with a very light color. In recent years, we have encountered this same difficulty when examining the mass-produced Dendrolimus punctatus polyhedrosis virus. Later through a variety of dye staining test, after repeated practice, found a simple staining method, are described below.