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目的:研究血清基质金属蛋白酶-8(MMP-8)、白细胞介素-6(IL-6)表达与缺血性脑血管病患者颈动脉粥样硬化斑块的关系。方法:选取2012年9月至2013年11月就诊60例缺血性脑血管病患者为研究组和健康体检者60例为对照组。按照超声影像学资料并参考患者颈动脉斑块类型把研究组分为不稳定斑块类型(18例),稳定斑块类型(22例)以及斑块性质介于两组之间为中间类型(20例)。采用双抗体夹心酶联免疫吸附试验(ELISA)对血清MMP-8、IL-6水平进行测定,探讨血清MMP-8、IL-6表达与不同程度缺血性脑血管病患者颈动脉粥样硬化斑块的关系。结果:研究组血清MMP-8、IL-6水平显著高于对照组,差异均有统计学意义(P<0.01);三种不同类型斑块血清中MMP-8、IL-6水平差异均有统计学意义(P<0.01);Spearman等级相关性分析发现,研究组患者颈动脉斑块稳定程度与血清MMP-8、IL-6浓度水平存在显著正相关关系(P<0.01);线性相关分析发现,研究组血清MMP-8水平与IL-6水平呈正相关关系(P<0.01)。结论:缺血性脑血管病患者血清MMP-8、IL-6水平明显升高,其与颈动脉斑块不稳定程度相关,同样与缺血性脑血管病发病机制方面存在相关协同作用。
Objective: To study the relationship between the expression of matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and carotid atherosclerosis in patients with ischemic cerebrovascular disease. Methods: From September 2012 to November 2013, 60 patients with ischemic cerebrovascular disease were selected as study group and 60 healthy subjects as control group. The study group was divided into unstable plaque type (18 cases), stable plaque type (22 cases) and plaque nature between the two groups according to the ultrasound imaging data and referring to the carotid plaque type 20 cases). Serum levels of MMP-8 and IL-6 were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to explore the relationship between the expression of serum MMP-8 and IL-6 and carotid atherosclerosis in patients with ischemic cerebrovascular disease Plaque relationship. Results: The levels of serum MMP-8 and IL-6 in the study group were significantly higher than those in the control group (P <0.01). The levels of MMP-8 and IL-6 in the three different types of plaque serum Statistically significant (P <0.01). Spearman rank correlation analysis found that there was a significant positive correlation between carotid plaque stability and serum MMP-8 and IL-6 levels in study group (P <0.01), and linear correlation analysis Found that the study group serum MMP-8 levels and IL-6 levels were positively correlated (P <0.01). Conclusion: Serum levels of MMP-8 and IL-6 in patients with ischemic cerebrovascular disease are significantly increased, which are correlated with the degree of instability of carotid plaques. There is also a synergistic effect in the pathogenesis of ischemic cerebrovascular disease.