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为了研究人类Ｈｂ Ｌｅｐｏｒｅ Ｂｏｓｔｏｎ基因结构及其表达低下的原因，该文用逆转录病毒为载体，克隆含不同珠蛋白基因５’－端启动子区的Ｌｅｐｒｏｅ基因和β基因，分别导入Ｋ_５６２细胞和Ｈｅｌａ细胞中，用竞争性逆转录ＰＣＲ技术检测其表达水平。结果发现：在Ｋ_５６２细胞中可能存在负调控因子或缺乏某种正调控因子，导致β珠蛋白基因无可检出的表达。在Ｈｅｌａｆｏ胞中，所导入的重组载体均有不同程度的表达。证明：δ基因５’端序列是导致Ｌｅｐｏｒｅ基因表达水平低下的重要原因，外显子１、内含子１对其影响甚微。以上结果为人类β－地中海贫血的基因治疗研究提供了有用的参考资料和理论基础。
In order to study the gene structure of Hb Lepore Boston and its low expression, Leproe and β genes containing the 5’-end promoter region of different globin genes were cloned into retroviral vector and introduced into K562 cells and Hela The cells were detected by competitive reverse transcription PCR. The results showed that there may be negative regulators or lack of some positive regulators in K562 cells, resulting in no detectable expression of beta globin gene. In Helafo cells, the recombinant vectors introduced had different degrees of expression. It is proved that the 5 ’end sequence of δ gene is an important reason leading to the low level of Lepore gene expression. Exon 1 and intron 1 have little effect on it. These results provide a useful reference and theoretical basis for the study of gene therapy of human β-thalassemia.