采用细胞生物学方法和组织培养技术研究小鼠胚胎原始生殖细胞(PGCs)的一般形态学特征及生物学特点.在体外培养条件下,PGCs 按体积大小可分大、中、小3类。本研究发现,中、小型原始生殖细胞在培养的第2、3 d 增多,形态似大型原始生殖细胞。培养中的 PGCs 不贴壁而悬浮生长于培养液中,培养到第6～7 d时开始死亡。体细胞(间充质细胞或间皮细胞)贴壁生长,生长旺盛,与PGCs 同时死亡。 The morphological characteristics and biological characteristics of embryonic primordial germ cells (PGCs) were studied by using cell biology and tissue culture techniques.Under the conditions of in vitro culture, PGCs could be divided into three groups according to their size. The study found that medium and small primordial germ cells increased in the first 2,3 days of culture, shaped like a large primitive germ cells. The PGCs in culture did not attach to the wall and grew in suspension in the culture medium until the sixth to seventh day of culture. Somatic cells (mesenchymal or mesothelial cells) adherent growth, vigorous growth, and PGCs died at the same time.