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为探索适宜生产莱氏野村菌分生孢子的培养基及培养条件,利用单因素试验对大豆、小麦、燕麦、大米等材料进行筛选,并优化发酵条件。结果表明,不同基质对莱氏野村菌分生孢子产生、孢子活力、抗逆性和毒力有明显影响。莱氏野村菌在大豆上的产孢量和孢子活力明显高于其它三种基质,但小麦上培养的孢子抗逆性和毒力明显高于其它处理。小麦预浸泡12h后进行发酵,产孢量比对照提高25.9%;小麦浸泡后粉碎成颗粒,可显著提高产孢量;在粉碎的麦粒中添加2/5～3/5(w/w)的谷壳增加其透气性,有利于分生孢子产生,产孢量比对照提高34.7%～42.0%。固体基质接种后的初始含水量和培养温度是影响产孢的重要因素,含水量为65%、培养温度为24～26℃适宜菌体生长和分生孢子产生。光照是分生孢子产生的必需条件,连续光照有利于分生孢子产生。
In order to explore the proper medium for the production of Conidiophora sp. And culture conditions, single factor experiments were used to screen soybean, wheat, oats and rice, and the fermentation conditions were optimized. The results showed that different substrates had significant effects on conidia generation, spore vigor, stress resistance and virulence of Lactarius. The spore yield and spore activity of N. nivalis on soybean were significantly higher than those on the other three substrates. However, the spore resistance and virulence of wheat cultivated on wheat were significantly higher than those of other treatments. The wheat was pre-soaked for 12h, and the sporulation amount was increased by 25.9% compared with the control. After soaking, the wheat was pulverized into granules, which could significantly increase the sporulation rate. Adding 2-5-3 / 5 (w / w) The increase of the permeability of the chaff is conducive to the production of conidia, sporulation than the control increased 34.7% ~ 42.0%. The initial water content and culture temperature after solid substrate inoculation is an important factor affecting sporulation. The water content is 65%, and the culture temperature is 24 ~ 26 ℃ for proper growth of the cells and conidia. Light is an essential condition for conidia, and continuous light is good for conidia production.