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目的研究艾叶多糖对I型糖尿病小鼠的降血糖作用。方法采用腹腔注射链脲佐菌素的方法建立I型糖尿病小鼠模型,灌胃给予糖尿病模型小鼠艾叶多糖(100,200和400 mg·kg-1·d-1),连续30d,观察糖尿病小鼠的血糖以及糖耐量的变化,比色法和ELISA法分别测定肝糖原以及血清胰岛素水平。灌胃给予健康大鼠艾叶多糖(100,200和400 mg·kg-1)后提取含药血浆,与大鼠胰岛细胞体外共孵育,ELISA法测定培养上清液中胰岛素含量。结果艾叶多糖能明显降低糖尿病小鼠的血糖浓度,改善糖耐量,还能显著增加肝糖原存储量(P<0.05),100和200 mg·kg-1·d-1剂量的艾叶多糖还能提高血清胰岛素水平(P<0.05,P<0.01),但艾叶多糖对胰岛细胞分泌胰岛素无影响。结论艾叶多糖能增加糖尿病小鼠的肝糖原、改善胰岛素分泌,调节血糖浓度。
Objective To study the hypoglycemic effects of leaves of Astragalus mongholicus on type I diabetic mice. Methods The mouse model of type I diabetes mellitus was established by intraperitoneal injection of streptozotocin (STZ). The diabetic mice (100, 200 and 400 mg · kg-1 · d-1) were given intragastrically for 30 days. Diabetic mice Of blood glucose and glucose tolerance changes, colorimetry and ELISA were measured glycogen and serum insulin levels. The medicated plasma was extracted from the leaves of healthy rats by intragastric administration of 100, 200 and 400 mg · kg-1, incubated with rat islet cells in vitro, and the content of insulin in the supernatant was measured by ELISA. Results Artemisia argyi polysaccharide can significantly reduce the diabetic mice blood glucose concentration, improve glucose tolerance, but also significantly increased hepatic glycogen storage (P <0.05), 100 and 200 mg · kg-1 · d-1 doses of leaves polysaccharide can Increase serum insulin level (P <0.05, P <0.01), but there is no effect of AI leaf polysaccharide on insulin secretion from islet cells. Conclusion Artemisia leaf polysaccharide can increase hepatic glycogen in diabetic mice, improve insulin secretion and regulate blood glucose concentration.