论文部分内容阅读
目的研究钙拮抗剂对急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)病程中前列环素的影响和对肾损害的保护作用。方法观察外源性钙拮抗剂(异搏定)对 ANP 合并肾损害时大鼠肾组织学改变,肾静脉血中血栓素 A_2(thomboxane A_2,TXA_2)和前列环素 I_2(prostaglandin I_2,PGI_2)的代谢产物比值,肾功能变化及动物病死率的影响。结果 ANP 模型制备后4h、8h 胰、肾出现严重损害,肾静脉血血栓素B_2(TXB_2)与6-酮-前列环素_1α(6-Keto-PGF_1α比值即 TXB_2/6-Keto-PGF_1α明显上升,肾功能明显下降;异搏定治疗组 ANP 大鼠肾静脉 TXB_2/6-Keto-PGF_1下降,BUN、Cr 下降。实验组4h、8h BUN 分别为18.4±1.7、25.7±1.3mmol/L;治疗组相应时间点 BUN 为10.2±1.4、14.9±2.6mmol/L;实验组4h、8h 两时间点 Cr为146±11、190±22μmol/L;治疗组两时间点 Cr 为131±10、109±20μmol/L。肾脏组织学改变明显好转,动物病死率下降,存活时间延长。实验组大鼠病死率为87.5%,存活时间为17.6±2.6h;治疗组大鼠病死率为62.5%,存活时间为19.8±2.6h,两组 P 值均小于0.05。结论异搏定能提高 PGI_2,抑制TXB_2/6-Keto-PGF_1α平衡紊乱。对 ANP 所致的肾脏损害具有保护作用。
Objective To investigate the effect of calcium antagonist on prostacyclin in the course of acute necrotizing pancreatitis (ANP) and its protective effect on renal damage. Methods The effects of exogenous calcium antagonist (verapamil) on the renal histological changes, thomboxane A_2 (TXA_2), and prostaglandin I_2 (PGI_2) levels in renal tissue of ANP rats with renal damage were observed. Metabolite ratio, changes in renal function and animal mortality. Results The pancreas and kidney of the ANP model were severely damaged 4 h and 8 h after preparation. The ratio of TXB_2 to 6-Keto-PGF_1α in renal vein was significantly higher than that of TXB_2 / 6-Keto-PGF_1α (P <0.05), while the levels of BUN and Cr in renal vein decreased significantly in experimental group (18.4 ± 1.7 and 25.7 ± 1.3mmol / L, P <0.05) The BUN of the treatment group at the corresponding time point was 10.2 ± 1.4 and 14.9 ± 2.6 mmol / L, respectively. Cr was 146 ± 11 and 190 ± 22 μmol / L at 4h and 8h in the experimental group, and Cr ± 131 ± 10,109 ± 20μmol / L. Kidney histological changes improved significantly, animal mortality decreased, survival time extended.The experimental group of rats mortality was 87.5%, the survival time was 17.6 ± 2.6h; the treatment group rats mortality was 62.5%, survival The time was 19.8 ± 2.6h, the P values of both groups were less than 0.05.Conclusion Verapamil can increase PGI_2 and inhibit the balance of TXB_2 / 6-Keto-PGF_1αtolerance, which has a protective effect on ANP-induced renal damage.