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目的 研究单纯疱疹Ⅰ型病毒 (HSV 1)感染后疾病的严重性与宿主遗传因素的关系 ;分析HSV 1感染后小鼠角膜中炎症细胞的亚群分布 ;检测角膜三层细胞中病毒抗原的表达及其临床意义。方法 以单克隆抗体和常规组织学技术作免疫组织化学研究。结果 HSV 1感染后疾病取决于宿主的遗传因素 ,在角膜基质炎中 ,T细胞对引发疾病起主导作用 ,炎症细胞的数量与抗原量相关 ,病毒抗原沉积于角膜三层细胞 ,而BLAB/c中的频率 ( 71%～ 10 0 % )高于C5 7BL/ 6( 11%～ 5 6% )。结论 实验性单纯疱疹性角膜基质炎的严重性取决于宿主的遗传因素 :在引发角膜基质炎过程中 ,T细胞起主导作用 ;基质层为HSV 1最主要沉积场所 ,炎症细胞与病毒抗原的频率和部位一致
Objective To study the relationship between the severity of the disease and host genetic factors after infection with herpes simplex virus type 1 (HSV 1), analyze the subpopulation distribution of inflammatory cells in the cornea of HSV 1 infected mice, and detect the expression of viral antigens in corneal three layers And its clinical significance. Methods Monoclonal antibodies and routine histological techniques were used for immunohistochemistry. Results The disease after HSV 1 infection depends on the genetic factors of the host. In corneal stromal cells, T cells play a leading role in triggering the disease. The number of inflammatory cells is related to the amount of antigen. The viral antigen is deposited on the corneal three layers of cells. BLAC / (71% ~ 100%) was higher than C5 7BL / 6 (11% ~ 56%). Conclusions The severity of experimental herpes simplex keratitis depends on the genetic factors of the host: T cells predominate during the initiation of corneal stromal inflammation; the stromal layer is the most important depositional site of HSV 1 and the frequency of inflammatory cells and viral antigens Consistent with the site