论文部分内容阅读
目的：观察鸡冠微血管在叶绿素光敏剂光动力治疗后的超微结构变化。方法：２４只动物随机分成对照组和治疗组，光动力治疗组治疗参数为：给药至照光间隔时间１０ｍｉｎ，光敏剂剂量每千克体重给１０ｍｇ，功率密度１５０ｍＷ／ｃｍ２，能量密度９０Ｊ／ｃｍ２，治疗光源为主发射峰位于６５０ｎｍ的红光。结果：透射电镜观察显示红细胞破坏，形态不规则，内皮细胞肿胀致血管腔变窄，胞膜破裂，线粒体肿胀、空泡化，内质网扩张，核染色质聚积或边集、核溶解，部分区域可见内皮细胞脱落，血管基膜及管周胶原纤维暴露，血栓形成。结论：内皮细胞是光动力损伤的主要靶部位，光动力效应产生的单态氧或氧自由基对内皮细胞造成直接光毒损伤，血管内层损伤导致凝血因子释放，血栓形成引起的缺氧加重、加速了血管组织的损伤。
OBJECTIVE: To observe the ultrastructural changes of capillaries capillaries after photodynamic therapy with chlorophyll photosensitizer. Methods: Twenty-four animals were randomly divided into control group and treatment group. The parameters of PDT group were as follows: administration to illumination interval 10 min, photosensitizer dosage 10 mg / kg body weight, power density 150 mW / cm2, energy density 90 J / Treatment of light-based emission peak located at 650nm red. Results: Transmission electron microscopy showed the destruction of erythrocytes, irregular morphology, narrowing of blood vessel cavity caused by swelling of endothelial cells, rupture of membrane, mitochondria swelling, vacuolization, endoplasmic reticulum dilation, nuclear chromatin accumulation or edge aggregation, nuclear lysis, Area visible endothelial cell shedding, vascular basement membrane and peri-collagen exposure, thrombosis. Conclusion: Endothelial cells are the main targets of photodynamic damage. The singlet oxygen and oxygen free radicals generated by photodynamic effect cause direct phototoxic injury to endothelial cells. The injury of vascular endothelium leads to the release of coagulation factors and the increased hypoxia caused by thrombosis , Accelerated the damage of vascular tissue.