作者们通过去除卵巢2周后雌鼠的脉冲式黄体生成素(LH)释放的变化,以鉴定处在持久动情(CE)状态雌鼠的 LH 分泌短缺的性质,然后用α-肾上腺素能的刺激来测验老年鼠的 LH 脉冲的幅度与频率的恢复能力。选择有规则的4或5天动情周期的幼鼠(鼠龄4～5月)、CE 的中龄鼠(鼠龄11～12月)与高龄鼠(鼠龄21～23月)作实验。后两者已分别处于CE 状态2～3月和6～10月,并一直保持着健康状况。实验:给幼鼠组,中龄鼠组及高龄鼠组的鼠均作了卵巢切除术,且在13天以后经颈静脉插入一个导管直到心房。次日通过此导管给200IU 的肝素 The authors identified the nature of LH secretion deficits in female rats in a long-lasting estrus (CE) state by observing changes in pulsed luteinizing hormone (LH) release in females two weeks after ovariectomy and then using alpha-adrenergic Stimulation was used to test the recovery of the amplitude and frequency of LH pulses in aged mice. Young rats (4 months to 5 months) with a regular 4 or 5 day estrous cycle were selected. Middle-aged mice (11-12 months of age) were used for experiments with older rats (21-23 months of age). The latter two have been in the CE state 2 ~ March and 6 ~ October, and has maintained a healthy state. Experiments: Mice in the young, middle and advanced age groups were ovariectomized, and a catheter was inserted through the jugular vein to the atrium after 13 days. The next day through this catheter to give 200IU of heparin