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把柑桔黄龙病(Citrus Huanglongbing,HLB)阳性核酸样品进行梯度稀释后,分别采用HLBp、CQULAP两组TaqMan水解探针法和以rpl基因为目的片段的SYBR Green染料法进行HLB实时荧光定量PCR(Quantitative Real-time PCR,qPCR)检测,结果发现,HLBp探针法可以从稀释105倍的阳性叶片核酸样品(病菌拷贝数约为60个)中检测到病原菌,高于CQULAP探针法和SYBR Green染料法。将3种qPCR检测体系用于赣州安远县、寻乌县和赣县送检的60份田间样品检测,结果发现,HLBp探针法的阳性率为86.7%,CQULAP探针的阳性率为60.0%,SYBR Green染料法的阳性率为68.3%。
After gradient dilution of Citrus Huanglongbing (HLB) -positive nucleic acid samples, the HLBp and CQULAP TaqMan hydrolysis probes and the SYBR Green dye method with rpl gene were used respectively to perform HLB real-time quantitative PCR Quantitative Real-time PCR (qPCR), we found that the pathogen was detected by the HLBp probe method from 105-fold dilutions of positive leaf nucleic acid samples (about 60 copies of the bacteria), higher than CQULAP probe method and SYBR Green Dye method. Three kinds of qPCR detection system were used to detect 60 field samples in Anyuan County, Xunwu County and Ganxian County, Ganzhou Prefecture. The results showed that the positive rate of HLBp probe method was 86.7% and the positive rate of CQULAP probe was 60.0 %, The positive rate of SYBR Green dye method was 68.3%.