毋庸置疑,恶性疟原虫配子体的分化和成熟,是一系列程序化的、发生在分子和细胞水平的特定事件。早期(Ⅰ、Ⅱ期)配子体即出现丰富的胞浆蛋白Pfg27/25、纳虫泡蛋白Pfg16和膜下微管;至Ⅲ期始有性分化的形态特征。雌配子体表达α-微管蛋白Ⅱ,雄配子体表达Pf77:Ⅳ期雌配子体膜下出现嗜锇小体,与之相关的是膜联蛋白Pfg377。作者采用免疫荧光双染技术,对配子体成熟和性分化过程中的Pfg377表达特征进行了分析。 Undoubtedly, the differentiation and maturation of P. falciparum gametocytes is a series of programmed events that occur at the molecular and cellular levels. The early (Ⅰ, Ⅱ) gametophytes showed rich cytoplasmic protein Pfg27 / 25, NPF Pfg16 and submucosal microtubules; Female gametophyte expressed α-tubulin Ⅱ, male gametophyte expression Pf77: Ⅳ stage female gametophyte membrane Osmotic Osmotic Osmosis body, which is associated with annexin Pfg377. The authors used immunofluorescence double staining technique to analyze the expression characteristics of Pfg377 during gametophytic maturation and sexual differentiation.