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目的:建立硬皮病小鼠模型,探讨抗硬化复方抗博莱霉素所致小鼠皮肤硬变的作用机理。方法:制作硬皮小鼠动物模型:博莱霉素(BLM)200μg/ml每日于C3H小鼠背部(剃毛后)皮下注射,连续3周。药物作用机理研究:运用免疫组织化学法测定硬皮小鼠模型皮肤中Ⅰ、Ⅲ型胶原和转化生长因子-β1(TGF-β1)等的表达,观察抗硬化复方对硬皮小鼠皮肤Ⅰ、Ⅲ型胶原和TGF-β1表达的影响。53只C3H鼠分成6组。①空白对照组;②造模对照组;③早服中药组,造模同时服用中药;④中药组,造模成功后服中药;⑤西药组,造模成功后服青霉胺;⑥中西药合用组。比较研究上述指标在各组中的变化,采用SPSS11.0统计软件进行分析。结果:抗硬化复方能对抗博莱霉素所致的小鼠皮肤硬变,早服中药组、中药组、西药组及中、西药组小鼠皮肤COL-Ⅰ、COL-Ⅲ免疫组织化学指数均明显低于造模对照组小鼠,差异有非常显著意义(P<0.01)。早服中药组及中西药合用组效果尤佳。早服中药组、中药组及中、西药合用组小鼠皮肤TGF-β1、COL-Ⅲ免疫组织化学指数又明显低于西药组小鼠,差异有非常显著意义(P<0.01)。结论:抗硬化复方能有效抑制硬皮小鼠皮肤中Ⅰ型胶原、Ⅲ胶原的合成,有抗组织纤维化作用。
Objective: To establish a mouse model of scleroderma and explore the mechanism of action of anti-sclerosis compound anti-bleomycin-induced mouse dermal cirrhosis. METHODS: Animal models of scleroderma were established: BLM 200 μg / ml was injected subcutaneously on the back of the C3H mice (shaved) daily for 3 weeks. Pharmacological mechanism: The expression of type I and type III collagen and transforming growth factor-β1 (TGF-β1) in the skin of scleroderma was determined by immunohistochemical method. Type Ⅲ collagen and TGF-β1 expression. 53 C3H mice were divided into 6 groups. ① control group; ② model control group; ③ early taking traditional Chinese medicine group, taking traditional Chinese medicine at the same time; ④ traditional Chinese medicine group, taking traditional Chinese medicine after successful model making; ⑤ Western medicine group, taking penicillamine after successful model making; Combined group. Comparative study of the above indicators in each group changes, using SPSS11.0 statistical software for analysis. Results: The anti-sclerotic compound can prevent bleomycin-induced mouse skin lesions. The immunohistochemical indexes of COL-Ⅰ and COL-Ⅲ in the skin of mice in the traditional Chinese medicine group, traditional Chinese medicine group, western medicine group, Significantly lower than the control group mice, the difference was significant (P <0.01). As early as traditional Chinese medicine group and Western medicine combined effect is better. Immunohistochemical index of TGF-β1 and COL-Ⅲ in the skin of mice treated with Chinese herbs and traditional Chinese medicine combined with Chinese herbs and Western medicine was significantly lower than that of Western medicine group (P <0.01). Conclusion: The anti-sclerotic compound can effectively inhibit the synthesis of type Ⅰ collagen and type Ⅲ collagen in the skin of scleroderma mice and has the effect of anti-tissue fibrosis.