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[目的]为研究植物根际与土壤酶活性及评价土壤环境质量提供理论依据。[方法]通过盆栽试验研究了Pb、Zn单独及复合污染对不同生长时期小麦根区、非根区土壤蔗糖酶活性的影响。[结果]Pb胁迫下,从幼苗期到抽穗期,根区蔗糖酶活性的增幅分别为401%、461%和444%,非根区增幅分别为365%、356%和405%;从抽穗期到成熟期,根区蔗糖酶活性的降幅分别为44%、53%和39%,非根区降幅分别为37%、67%和58%;幼苗期根区蔗糖酶活性分别下降了67%、72%和72%,非根区分别下降了68%、69%和72%。Zn胁迫下,根区蔗糖酶活性的最大值与最小值之比分别为8.87、16.45和7.35,非根区分别为7.25、17.04和15.24。在Pb-Zn胁迫下,根区蔗糖酶活性的最大值分别为178.558、175.659和254.752 mg/g,非根区分别为163.237、160.338和149.986 mg/g。[结论]在小麦生长过程中,Pb、Zn胁迫下根区蔗糖酶活性高于非根区。
[Objective] The research aimed to provide theoretical basis for studying plant rhizosphere and soil enzyme activities and evaluating soil environmental quality. [Method] Pot experiments were conducted to study the effects of Pb and Zn alone and combined pollution on the soil invertase activities in the root zone and non-root zone of wheat at different growth stages. [Result] Under Pb stress, the increase of invertase activity in root zone was 401%, 461% and 444% respectively from the seedling stage to the heading stage, while the increment in non - root zone was 365%, 356% and 405% respectively. At the mature stage, the sucrosease activity in the root zone decreased by 44%, 53% and 39%, respectively, and decreased by 37%, 67% and 58% in the non-root zone respectively. The sucrase activity in the root zone decreased by 67% 72% and 72% respectively, while the non-root zone decreased by 68%, 69% and 72% respectively. Under Zn stress, the ratio of maximum to minimum value of sucrase activity in root zone was 8.87, 16.45 and 7.35, respectively, and that in non-root zone was 7.25, 17.04 and 15.24, respectively. Under Pb-Zn stress, the maximum sucrase activity in the root zone was 178.558, 175.659 and 254.752 mg / g, respectively, and the non-root zone was 163.237, 160.338 and 149.986 mg / g, respectively. [Conclusion] In the process of wheat growth, sucrase activity in root zone under Pb and Zn stress was higher than that in non-root zone.