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本研究用酶联免疫吸附术观察8只家兔在附睾注射鱼肝油酸钠后血清及精浆中的抗精子抗体。血清抗精子抗体阳性率为37.5～50.0%,最早检出率为术后2个月,6～7月达高峰。精浆抗精子抗体阳性率为25%,最早检出率为术后4个月,且此时己达高峰。与输精管结扎资料相比,发现血清抗精子抗体检出率偏低,而精浆相应抗体偏高,表明向附睾注射鱼肝油酸钠引起的免疫反应有别于结扎,以生殖道局部为主。
In this study, anti-sperm antibodies in serum and seminal plasma of eight rabbits were detected by enzyme-linked immunosorbent assay (ELISA) in the epididymis injected with sodium morrhuate. Serum anti-sperm antibody positive rate was 37.5 ~ 50.0%, the earliest detection rate was 2 months after surgery, peaked in June to July. Seminal plasma anti-sperm antibody positive rate was 25%, the earliest detection rate was 4 months after surgery, and at this time has reached its peak. Compared with the data of vasectomy, we found that the detection rate of serum anti-sperm antibody was low, but the corresponding antibody of seminal plasma was higher, indicating that the immune response caused by injecting sodium morrhuate to the epididymis was different from that of ligation.