目的探讨非那雄胺抑制前列腺癌细胞增殖的分子机制,筛选特异性的治疗靶点。方法利用基因芯片技术检测非那雄胺作用前列腺癌细胞株前后LNCaP差异表达的基因。结果非那雄胺能够显著抑制前列腺癌细胞株LNCaP的增殖,在96条基因中受非那雄胺作用有差异表达的基因有29条,其中表达上调的基因有11条,下调的基因有18条。结论非那雄胺抑制LNCaP增殖可能涉及多基因、多通路的共同作用。 Objective To investigate the molecular mechanism of finasteride inhibiting the proliferation of prostate cancer cells and to screen specific therapeutic targets. Methods The genes of differentially expressed LNCaP before and after treatment with finasteride in prostate cancer cell lines were detected by gene chip technique. Results Finasteride could significantly inhibit the proliferation of prostate cancer cell line LNCaP. Among the 96 genes, 29 were differentially expressed by finasteride, of which 11 were up-regulated and 18 were down-regulated Article. Conclusion Finasteride inhibition of LNCaP proliferation may involve multi-gene, multi-pathway interaction.