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我利用500毫升医用吊瓶,将灵芝培养基装于瓶底部,高约5厘米左右,压实,在中心扎孔。将瓶口用棉塞或双层牛皮纸扎紧,然后进行高压灭菌。灭菌后待其冷却到室温,将灵芝菌种在无菌条件下接种到培养基的中心孔里。在环境温度为26～28℃的条件下进行培养,经7～10天后,培养面上全部长满菌丝体,12～15天菌丝体长满全部培养基。此时可将瓶口棉塞摘去,环境湿度要保持在90～95％。这样子实体原基发生并随之而开始分化,由白色→淡黄色→深黄色,同时长出菌柄。菌柄分支,稀奇古怪,可供观赏。经50～60天后,即可认定成熟。但因瓶内没有空气自然流动的条件,菌柄并不开伞。注入60度的粮食白酒,菌柄即停止生长,菌柄因酒浸泡变成深褐色,增加观赏价值。浸泡15～20天后,酒液呈深褐色,即可饮用,有灵芝药酒的同等作用。因笔者缺乏摄影条件,未能拍下照片。现已培养出20多瓶,菌柄形态各异。
I use 500 ml medical bottle, the Ganoderma lucidum culture medium installed in the bottom of the bottle, about 5 cm high, compaction, hole in the center. Tighten the bottle with tampon or double kraft paper and autoclave. After sterilization, allow to cool to room temperature, Ganoderma lucidum strains were inoculated into the center hole of the medium under aseptic conditions. The culture was carried out at an ambient temperature of 26 to 28 ° C. After 7 to 10 days, all of the culture surface was covered with mycelium and the entire mycelium was covered with the culture medium for 12 to 15 days. At this point can be removed tampons bottle, the ambient humidity to maintain at 90 ~ 95%. This occurs when the entity primordium begins to differentiate with it, from white → light yellow → dark yellow, while growing stipe. Stipe branch, bizarre, for viewing. After 50 to 60 days, you can be considered mature. But because of the bottle there is no natural flow of air conditions, stipe does not open the umbrella. Into 60 degrees of food liquor, the stigma that stop growing, stigma due to the wine into a dark brown, increase ornamental value. Soak 15 to 20 days later, dark brown wine, you can drink, have the same effect of Ganoderma wine. Because I lack of photography conditions, failed to take pictures. Has now cultivated more than 20 bottles, stipe shape.