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目的 :建立一种测定利多卡因代谢产物单乙基甘氨酰二甲苯胺 (MEGX)含量的高效毛细管电泳法。方法 :用50mmol/L硼酸缓冲液 (pH =8.8)为电泳电解液 ,电压 1 2kV ,运行电流 1 8.8～ 2 3 .2 μA ,检测波长 2 1 4nm ,有效毛细管长度 50cm ,管径 50 μm ,氨茶碱为内标 ,展开 1 2min。 8只昆明种小白鼠腹腔注射 0 .2 %利多卡因 4 0mg/kg ,用本法测定其 5h尿液中MEGX累积排泄量。结果 :MEGX迁移时间 7.8min ,氨茶碱迁移时间 1 0 .7min。MEGX含量为 0 .0 62～1 .0mg/ml时 ,其浓度与色谱峰面积线性关系良好 ,r =0 .9991 ,日内、日间变异系数均小于 7.8% ,回收率为98.4 %～ 1 0 1 .6% ,常用 3 0种药物对其无干扰。 8只小鼠MEGX累积排泄量为 ( 2 95.2± 55.2 ) μg ,其中游离型MEGX为 4 4.7% ,结合型为 55.3 %。结论 :该方法简单、快速、灵敏 ,特异性和重现性良好 ,具有较强实用价值。
OBJECTIVE: To establish a high performance capillary electrophoresis method for the determination of the content of monoethylglycyldimethyl aniline (MEGX) in the metabolite of lidocaine. Methods: 50 mmol / L boric acid buffer (pH = 8.8) was used as the electrophoresis electrolyte. The voltage was 12 kV, the operating current was 8.82.22 μA, the detection wavelength was 2 14 nm, the effective capillary length was 50 cm, the diameter was 50 μm, Aminophylline for the internal standard, start 12min. 8 Kunming mice were intraperitoneally injected 0.2% lidocaine 40mg / kg, using this method to determine the cumulative excretion of MEGX 5h urine. Results: MEGX migration time 7.8min, aminophylline migration time 10.7min. The concentration of MEGX was 0. 62 ~ 1 .0mg / ml, its concentration and peak area of a good linear relationship, r = 0.9991, day and day coefficient of variation were less than 7.8%, the recovery rate was 98.4% ~ 10 1 .6%, commonly used 30 kinds of drugs on its non-interference. The cumulative excretion of MEGX in 8 mice was (2 95.2 ± 55.2) μg, with 4.7% of free MEGX and 55.3% of combined MEGX. Conclusion: The method is simple, rapid, sensitive, specific and reproducible, with strong practical value.