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目的:观察维A酸对HL-60细胞增殖与NF-κB、VEGF表达的影响,以探讨维A酸治疗白血病的作用机制。方法:将不同浓度的维A酸作用于HL-60细胞72h后,采用MTT法观察细胞生长,用ELISA法检测上清液中VEGF的含量,并用流式细胞术检测NF-κB活性表达及细胞周期。结果:HL-60细胞经维A酸作用72h后生长显著受抑制,实验组HL-60细胞上清液中VEGF含量、NF-κB表达下降,10-6M、10-7M组与对照组相比差异显著(P<0·05),而10-8M组与对照组比较,无显著差异(P>0·05),其中10-6M组NF-κB表达与VEGF含量呈显著正相关(r=0·55,P<0·01,n=6),各治疗组G1期细胞明显升高,10-6M组、10-7M组与对照组相比,差异显著(P<0·01),而10-8M组与对照组比较,无显著差异(P>0·05)。结论:维A酸对HL-60细胞增殖有抑制作用,其作用机制可能与NF-κB活性及VEGF表达水平下调有关。
OBJECTIVE: To observe the effect of tretinoin on the proliferation of HL-60 cells and the expression of NF-κB and VEGF in order to investigate the mechanism of Tretinoin-induced leukemia. METHODS: HL-60 cells were treated with different concentrations of retinoic acid for 72 hours. The cell growth was observed by MTT assay. The content of VEGF in the supernatant was detected by ELISA. The expression of NF-κB and the expression of NF-κB in cells were detected by flow cytometry cycle. Results: The growth of HL-60 cells was inhibited significantly after treated with Tretinoin for 72 hours. The content of VEGF and the expression of NF-κB in HL-60 cell supernatant of experimental group were decreased. Compared with control group, (P <0.05), but there was no significant difference between 10-8M group and control group (P> 0.05). The expression of NF-κB in 10-6M group was positively correlated with VEGF level (r = 0.05, P <0.01, n = 6). The cells in G1 phase in each treatment group were significantly higher than those in control group (P <0.01) However, there was no significant difference between 10-8M group and control group (P> 0.05). CONCLUSION: Tretinoin inhibits the proliferation of HL-60 cells, and its mechanism may be related to the decrease of NF-κB activity and VEGF expression.