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目的:研究C57-TgN(HBVadr2.0)SMMU“3号”品系乙型肝炎转基因小鼠肝脏的组织病理学及部分免疫学特征。方法:通过对130例SPF级乙型肝炎转基因小鼠和30例同龄正常C57BL/6小鼠肝组织进行免疫组织化学染色、HE染色和浸银染色分析,研究转基因小鼠HBsAg的表达以及病理改变情况。选取15例肝组织有单个核细胞浸润的转基因小鼠检测白细胞分化抗原的表达情况。结果:60.77%(79/130)的转基因小鼠肝脏组织中出现了不同程度的肝组织病理性改变,包括肝细胞变性、单个核细胞浸润和纤维组织增生,且与月龄正相关,而与性别、病毒蛋白的表达无相关性。选取出现单个核细胞浸润的肝组织进行免疫组织化学检测发现,肝组织中浸润的单个核细胞大多为CD3+、CD4+T细胞。结论:C57-TgN(HBVadr2.0)SMMU“3号”品系乙肝全基因组转基因小鼠肝脏产生类似慢性无症状HBV携带者的病理学变化,病理改变与病毒蛋白表达无相关性,与小鼠月龄呈正相关性。
Objective: To study the histopathology and partial immunological features of liver in transgenic mice with C57-TgN (HBVadr2.0) SMMU “3” strain. Methods: The expression of HBsAg and its pathological changes in transgenic mice were studied by immunohistochemical staining, HE staining and silver staining staining in 130 SPF hepatitis B transgenic mice and 30 normal-aged C57BL / 6 mice. Happening. Fifteen transgenic mice with mononuclear cell infiltration in liver tissue were selected to detect the expression of leukocyte differentiation antigen. Results: There were pathological changes of hepatic tissue in liver tissues of 60.77% (79/130) transgenic mice, including degeneration of hepatocytes, infiltration of mononuclear cells and fibrosis, which were positively correlated with age, Sex, viral protein expression no correlation. Immunohistochemical detection of liver tissue with mononuclear cell infiltration revealed that most of the infiltrating mononuclear cells in liver tissue were CD3 + and CD4 + T cells. Conclusion: There is no correlation between pathological changes and viral protein expression in the liver of C57-TgN (HBVadr2.0) SMMU “3” strain transgenic mice with chronic asymptomatic HBV infection Rat age was positively correlated.