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本文研究了温度变化对刺参(Apostichopusjaponicus)免疫和抗氧化指标的影响,实验设置为:对照组(CT,14℃)、慢速升温组(ST,14~20℃)和快速升温组(LT,14~26℃),取样时间为0、3、6、9、12、15、20d,分别取刺参体腔液、体壁和呼吸树测定免疫防御和抗氧化指标。结果表明:温度变化对刺参免疫防御和抗氧化指标影响显著(P<0.05)。各处理组总体腔细胞数量、吞噬率以及体腔液溶菌活力在3~12d内呈峰值变化,于第9d时达到最大值,至12d分别恢复至或显著低于对照组水平。而ST和LT抗菌活力、凝集活性分别在15、12d后显著低于对照组水平。各处理组体腔液总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性在15d内显著下降,而后维持稳定;各处理组体壁T-AOC活力、SOD活性、谷胱甘肽(GSH)含量和谷胱甘肽/氧化性谷胱甘肽(GSH/GSSG)在15d内逐渐上升,而后恢复至对照组水平;各处理组呼吸树T-AOC能力、SOD活性、GSH含量和GSH/GSSG逐渐上升,并于15d后维持稳定。由此可见,温度变化会在一定时间内引起刺参免疫应激反应,而随着温度的升高刺参免疫防御功能受到明显抑制,并且刺参不同组织应对温度变化的抗氧化响应机制不同,抗氧化水平为呼吸树>体壁>体腔液,随着温度的升高刺参整体抗氧化水平受到抑制。
In this paper, the effects of temperature on the immune and antioxidant indexes of Apostichopus japonicus were studied. The experimental settings were: control group (CT, 14 ℃), slow heating group (ST, 14 ~ 20 ℃) and fast heating group , 14 ~ 26 ℃). The sampling time was 0, 3, 6, 9, 12, 15 and 20 days. The immune defense and anti-oxidation indexes were determined by the body cavity fluid, The results showed that the temperature had significant effects on the immune defense and antioxidation of sea cucumber (P <0.05). The total number of luminal cells, phagocytic rate and body fluid lysozyme activity in each treatment group peaked within 3 ~ 12d, reaching the maximum value on the 9th day and returned to or significantly lower than that of the control group on the 12th day respectively. The antibacterial activity and agglutination activity of ST and LT were significantly lower than those of the control group at 15 and 12 days respectively. The total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity in body cavity fluid of each treatment group decreased significantly within 15 days and then maintained stable. T-AOC activity, SOD activity, (GSH) and glutathione / glutathione (GSH / GSSG) increased gradually within 15 days and then returned to the control level. The T-AOC ability, SOD activity and GSH content And GSH / GSSG increased gradually and remained stable after 15 days. Thus, the temperature changes will cause immune response to sea cucumber during a certain period of time, but with the increase of temperature, the immune defense function of sea cucumber is significantly inhibited, and the different mechanisms of different tissues of sea cucumber to respond to temperature changes are different, Antioxidant levels were respiratory tree> body wall> body cavity fluid, with the increase of temperature, the anti-oxidation level of S. japonicus was inhibited.