草甘膦是一种杀草谱广、低毒、土壤残留量低的灭生性除草剂[1]。但长期单一使用,可导致抗(耐)草甘膦杂草的产生,对农业生产造成严重损失[2]。目前检测杂草对草甘膦敏感性的普遍方法有整株植物测定法和培养皿法等[3],但都需要采集种子培育,而且一些种子还具有休眠时间长且难以打破的特点,因此确定敏感性需要较长时间。本研究以农田常见的5种杂草为材料,在室内及田间条件下,探 Glyphosate is a biocidal herbicide with broad spectrum of herbicides, low toxicity and low residual amount of soil [1]. However, long-term single use can lead to the generation of resistant (resistant) glyphosate weeds, causing serious losses to agricultural production [2]. At present, the common methods for detecting the sensitivity of weeds to glyphosate are the whole plant assay and the Petri dish method [3], but all require the collection of seed and some of the seeds also have the characteristics of long dormancy and hard to be broken. Therefore, It takes a long time to determine the sensitivity. In this study, five kinds of common weed in farmland were used as materials to explore under indoor and field conditions