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目的探讨肿瘤细胞分化诱导剂尿多酸肽(CDAⅡ)对乳腺癌细胞周期分布和CyclinD1表达的的影响。方法将CDAⅡ与不同生物学特性的乳腺癌细胞株MCF7和MDAMB231进行体外培养,分成3组:对照组、CDAⅡ组和维甲酸(ATRA)组,观察CDAⅡ对乳腺癌细胞生长曲线、细胞周期、CyclinD1表达及形态学等方面的影响。结果CDAⅡ可减缓2株乳腺癌细胞的生长和增殖能力,改变细胞周期分布,使MCF7细胞株对照组、CDAⅡ组和ATRA组S期比例分别为33.04%、21.70%和23.64%;MDAMB231细胞分别为38.7%、28.7%和27.9%;MCF7细胞株对照组、CDAⅡ组和ATRA组细胞CyclinD1表达(荧光强度)分别为12.26、6.46和6.74,MDAMB231细胞分别为12.43、8.32和8.97。结论CDAⅡ可抑制不同生物学特性的2株乳腺癌细胞的生长和增殖能力,使细胞周期出现G0/G1期阻滞,并减少乳腺癌细胞CyclinD1表达。
Objective To investigate the effect of CDA Ⅱ on the cell cycle distribution and the expression of CyclinD1 in breast cancer cells. Methods CDAⅡand breast cancer cell lines MCF7 and MDAMB231 with different biological characteristics were cultured in vitro and divided into three groups: control group, CDAⅡgroup and ATRA group. The effects of CDAⅡon the growth curve, cell cycle, CyclinD1 Expression and morphology and other aspects of the impact. Results CDAⅡ could slow down the growth and proliferation of two breast cancer cells and change the cell cycle distribution. The proportion of S phase in MCF7 cell line, CDAⅡgroup and ATRA group was 33.04%, 21.70% and 23.64% respectively. The MDAMB231 cells were 38.7%, 28.7% and 27.9% respectively. The expression of CyclinD1 (fluorescence intensity) in control group, CDAⅡgroup and ATRA group were 12.26,6.46 and 6.74 respectively, and MDAMB231 cells were 12.43,8.32 and 8.97 respectively. Conclusion CDAⅡ can inhibit the growth and proliferation of two breast cancer cells with different biological characteristics, block G0 / G1 arrest in the cell cycle and decrease the expression of CyclinD1 in breast cancer cells.