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为探明亚铁氰化物在植物体内的迁移、转化及对植物的毒性作用,以长出新根须和嫩叶的垂柳(SalixbabylonicaL.)枝条为材料,在自行设计的250ml生物反应器中生长192h,培养温度为24.0±1℃,亚铁氰化物水溶液的浓度分别为52.99,105.98,211.95和317.93mgCNL-1。结果表明:(1)低浓度实验组(52.99mgCNL-1)水溶液中10.85%的亚铁氰化物被植物吸收,随着浓度的升高吸收到植物体内的亚铁氰化物的比例(%)依次递减,但是统计学分析显示各实验组单位体重(湿重)的植物吸收亚铁氰化物的量无显著性差异;(2)在植物的各个部位都能检测到微量的亚铁氰化物,表明亚铁氰化物通过植物的蒸腾作用在植物体内的迁移。由于没有检测到在气态下的总氰化物,表明植物的蒸腾作用没有将亚铁氰化物释放到大气中;(3)尽管植物吸收到体内的亚铁氰化物是有限的,但物质平衡实验证明其在植物体内迁移的过程中超过96%的都能被植物有效转化;(4)所用的4种亚铁氰化物浓度在192h内没有对柳树产生毒性作用。因此认为:依据亚铁氰化物在水溶液→植物→空气系统内的迁移和转化,亚铁氰化物的植物修复是可能的。
In order to find out the migration, transformation and phytotoxicity of ferrocyanide in plants, the leaves of Salixbabylonica L. growing from new roots and leaves were grown in a self-designed 250 ml bioreactor 192h, the culture temperature was 24.0 ± 1 ℃, and the concentrations of ferrocyanide aqueous solution were 52.99,105.98,211.95 and 317.93mgCNL-1, respectively. The results showed that: (1) 10.85% of the ferrocyanide was absorbed by the plant in the low-concentration experimental group (52.99mgCNL-1) aqueous solution, and the proportion of ferrocyanide absorbed into the plant But statistical analysis showed that there was no significant difference in the amount of ferrocyanide absorbed by plants in each experimental group at body weight (wet weight); (2) traces of ferrocyanide were detected in all parts of the plant, indicating Migration of ferrocyanide in plants through transpiration of plants. The absence of total cyanide in the gaseous state indicates that transpiration of the plant did not release ferrocyanide to the atmosphere; (3) although the amount of ferrocyanide the plant absorbed into the body was limited, the mass balance experiment demonstrated More than 96% of its transformation in plants can be effectively transformed by plants. (4) The concentration of 4 ferrocyanides used has no toxic effect on willow in 192h. Therefore, it is considered that the phytoremediation of ferrocyanide is possible due to the migration and conversion of ferrocyanide in aqueous solution → plant → air system.