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目的 了解巴尔通体 (Bartonella)在云南鼠群中的分布及流行特征。方法 被检鼠血为 1999年 10月收集自云南省的 3个调查地区 ,采用兔血心浸液琼脂培养基进行巴尔通体分离 ,以聚合酶链反应 (PCR)对枸橼酸合酶基因 (gltA)的379bp片段进行扩增以证实是否巴尔通体 ,阳性者行以序列测定并与已知菌株加以比较。 结果与结论 从 131份鼠血分离到5 8株巴尔通体 (44 3% )。菌株分布于各调查点 ,感染鼠分属 3个属 6个种 ,以姬鼠属 (Apodemus)的带菌率最高 (6 2 2 % ,2 8/4 5 ) ,家鼠属 (Rattus)次之 (41 5 % ,2 7/ 6 5 ) ,绒鼠属 (Eothenomys)居第三位 (18 8% ,3/ 16 ) ,表明巴尔通体在云南常见鼠种中广泛分布及高度流行。所有菌株按其分离鼠属可分为 3群 ,具有以属为水平的宿主特异性。依基因结构可将它们分为 2 0个变异体 (家鼠属 8个 ;姬鼠属 12个 ;绒鼠属 2个 ) ,其中 17个为新发现变异体 ,表明云南巴尔通体基因型别的多样性。 7个家鼠巴尔通体变异体可分为B elizabethae、B tribocorum和新种B yunnannensis 3个基因型。由于云南巴尔通体的高度流行及基因型别的多样性 ,一些不明原因的疾病可能与巴尔通体的感染有关。需要对该地区巴尔通体的流行情况及对人类致病作用进行系统的调查和研究
Objective To understand the distribution and epidemiological characteristics of Bartonella in Yunnan rat population. Methods The rat blood was collected from three investigation areas collected from Yunnan Province in October 1999. The isolation of Bartonella was carried out by using rabbit blood heart infusion agar medium and the effects of citrate synthase gene gltA) of 379bp fragment was amplified to confirm whether Bartonella positive line with the determination of the sequence and compared with the known strains. RESULTS AND CONCLUSIONS Fifty eight Bartonella species (44.3%) were isolated from 131 rat blood samples. The strains were distributed at all survey sites, and the infected mice belonged to 3 genera and 6 species, with the highest infection rate of Apodemus (62.2%, 28/45), followed by Rattus (41.5%, 27/65) and Eothenomys (18.8%, 3/16), indicating that Bartonella is widely distributed and highly endemic in Yunnan common species. All strains according to their isolation can be divided into 3 groups of rats, with genus level of host specificity. According to the gene structure, they can be divided into 20 variants (8 in the genus Muscat; 12 in the genus Apodemus; 2 in the genus Secterus), of which 17 are newly discovered variants, indicating that the genotype Diversity. Seven Bartholin genera can be divided into three genotypes, B elizabethae, B tribocorum and a new species B yunnannensis. Due to the high prevalence and genotypic diversity of Bartonella in Yunnan, some unknown causes of the disease may be related to the infection of Bartonella. There is a need to systematically investigate and study the prevalence of Bartonella in the area and the human pathogenicity