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紫花苜蓿是异花授粉的四倍体植物,通过种子繁殖难以获得优良性状一致的后代群体,扦插作为一种无性繁殖手段,对苜蓿保优传代和加速繁殖扩大良种种群、培育良种具有重要的意义。本实验以选育的转基因苜蓿株系为试材,对影响苜蓿扦插成活的因素进行了系统研究,发现春季扦插效果好于秋季,现蕾初期的转基因苜蓿扦插成活率最高,达到了97.2%;来自于茎的基部和中部的插穗成活率高于顶端的插穗,分别达到了94%和100%;不同的株系之间的扦插成活率差异不大,新梢高度却有明显的区别,最高的达到了24.91cm;插穗留叶数的多少对扦插成活率和新梢的高度影响不大,但留叶数多的插穗新生叶片颜色比留叶数少的要绿;上部5cm细砂,底部5cm草炭土、珍珠岩、蛭石混合物的基质更利于插穗的成活,成活率最高达到了97.2%。
Alfalfa is cross-pollination of tetraploid plants, it is difficult to obtain the fine traits of offspring through seed multiplication. Cutting as a means of asexual reproduction, it has important significance for alfalfa to ensure superior passage and accelerated reproduction to expand the breeding population and cultivate the improved varieties . In this experiment, the transgenic alfalfa lines were used as tested materials to study the factors affecting the survival of alfalfa cutting. The results showed that the cutting effect in spring was better than that in autumn, and the survival rate of transgenic alfalfa reached 97.2% at the early budding stage. The survival rate of the cuttings from the base and the middle of the stems was higher than that of the top cuttings, reaching 94% and 100% respectively. There was no significant difference in the survival rates of cutting between the different strains, but the height of the shoots was obviously different from the highest ones Reached 24.91cm. The number of cuttings leaves had little effect on the survival rate of cuttings and the height of shoots, but the number of cuttings with more leaved leaves was less green than the number of leave leaves. The top 5cm fine sand and bottom The matrix of 5cm peat soil, perlite and vermiculite is more conducive to the survival of cuttings, the highest survival rate reached 97.2%.