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目的研究TRAIL联合顺铂诱导HOS-8603细胞的凋亡及其机制。方法 MTT法分别测定TRAIL,顺铂,和TRAIL+顺铂对HOS-8603细胞的抑制率,流式细胞法测定亚G1期细胞百分率及PI和Rh123双染色后细胞的ΔΨm。结果三组分别由TRAIL、顺铂、TRAIL+顺铂处理过的HOS-8603细胞用MTT法测抑制率分别为29%、33.6%、58.5%。随着药物作用时间增加,HOS-8603细胞凋亡数增加和ΔΨm降低(P<0.01),两者呈直线相关。结论 TRAIL和顺铂能协同诱导HOS-8603细胞凋亡,其机制可能是通过使线粒体膜通透性转运孔开放,ΔΨm降低来实现的。
Objective To study the apoptosis of HOS-8603 cells induced by TRAIL combined with cisplatin and its mechanism. Methods The inhibitory rates of TRAIL, cisplatin, and TRAIL + cisplatin on HOS-8603 cells were determined by MTT assay. The percentage of cells in sub-G1 phase and the ΔΨm of cells after PI and Rh123 double staining were determined by flow cytometry. Results The inhibitory rates of three groups of HOS-8603 cells treated with TRAIL, cisplatin and TRAIL + cisplatin were 29%, 33.6% and 58.5%, respectively. The apoptosis of HOS-8603 cells increased and the ΔΨm decreased (P <0.01) with the increase of the drug action time. The correlation between them was linear. Conclusion TRAIL and cisplatin can synergistically induce the apoptosis of HOS-8603 cells. The mechanism may be through the opening of the mitochondrial membrane permeability transport pore and the reduction of ΔΨm.