论文部分内容阅读
目的:研究大肠癌患者的细胞免疫功能及外科手术对它的影响。方法:分别应用APAAP法和乳酸脱氢酶释放法测定了43例大肠痼患者手术前后的外周血T淋巴细胞亚群和NK细胞(NKCC)活性。结果:患者术前CD_3、CD_4、CD_4/CD_8比值和NKCC分别为53.79±5.39,35.74±6.09,1.21±0.14,17.09±8.89,较对照明显降低,CD_8明显升高,为29.67±5.27;接受围手术期免疫治疗的10例患者,术后1周CD_3、CD_4、CD_4/CD_8比值及NKCC较术前明显刀高;而对照组术后2周内各指标除NKCC有升高外,余无明显变化,至4~6周各指标达正常水平。结论:说明术后2周内患者仍处于免疫抑制期,围手术期免疫治疗可提高患者的围手术期细胞免疫功能,缩短术后的免疫抑制期。
Objective: To study the cellular immune function of patients with colorectal cancer and the effect of surgery on it. METHODS: Peripheral blood T-lymphocyte subsets and NK cell (NKCC) activity were measured in 43 patients with colorectal fistula by APAAP method and lactate dehydrogenase release method, respectively. RESULTS: Preoperative CD3, CD4, CD4/CD8 ratio and NKCC were 53.79±5.39, 35.74±6.09, 1.21±0.14, and 17.09±8.89, respectively, which were significantly lower than those of the control, and the CD8 was significantly increased, which was 29.67±5.27. In the 10 patients who underwent immunotherapy during the surgical period, CD_3, CD_4, CD_4/CD_8 ratio and NKCC were significantly higher than those before surgery at 1 week postoperatively; whereas in the control group, except for NKCC, there was no significant increase in the index within 2 weeks after operation. Change, to 4 to 6 weeks, the indicators reached normal levels. Conclusions: The patient is still in immunosuppressive state within 2 weeks after surgery. Perioperative immunotherapy can improve the patient’s perioperative cellular immune function and shorten the postoperative immunosuppression period.