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目的:建立抗Thy1系膜增生性肾炎动物模型。方法:利用Ox-7杂交瘤细胞株产生抗Thy1抗体,纯化后尾静脉注射SD大鼠建立抗Thy1系膜增生性肾炎动物模型,不同时间点(0、3、5、7、10、14天)检测肾功能和尿蛋白定量,留取肾组织做病理,提取总蛋白。结果:抗Thy1肾炎模型组大鼠与对照组大鼠相比,第5天和第7天24小时尿蛋白定量显著升高(p<0.05,n=6),第10天后24小时尿蛋白定量开始下降,与系膜增殖变化程度一致。结论:本研究成功制备了抗Thy1肾炎大鼠模型,为研究系膜增生性肾炎提供了新的方法。
Objective: To establish an animal model of anti-Thy1 mesangial proliferative glomerulonephritis. Methods: The anti-Thy1 antibody was produced by Ox-7 hybridoma cell line. SD rat was injected into tail vein to establish an anti-Thy1 mesangial proliferative glomerulonephritis model. At different time points (0, 3, 5, 7, ) Detection of renal function and urinary protein quantitation, left renal tissue pathology, total protein extraction. Results: Compared with the control group, urinary protein in the model group was significantly increased (P <0.05, n = 6) on the 5th day and the 7th day on the 5th day and on the 7th day, and the urinary protein was quantified 24 hours after the 10th day Began to decline, and the proliferation of mesangial changes consistent. Conclusion: This study successfully prepared a rat model of anti-Thy1 nephritis, and provided a new method for studying mesangial proliferative glomerulonephritis.