液体菌种有生产周期短、菌龄一致,接于固体培养料发菌快的优点,但是一般都需设备。在没有发酵罐和摇床的情况下怎样生产出液体菌种?笔者曾进行过多次试验,现将一种简易有效的方法介绍如下: (一)母种制备 采用王梓英等的制备法,培养基为杂木屑88％,葡萄糖10％,蛋白栋1％,酵母粉1％,含水55％。装管后轻轻压实,并在中间打一孔再灭菌、接种,一般培养12天左右菌丝即可长满管。 (二)液体原种制备 培养基为玉米粉5.5％(过40目筛),葡萄糖0.5％,磷酸二氢钾0.1％,硫酸镁0.05％,维生0o.01％。搅拌匀后装入克氏瓶或茄形 Liquid bacteria have a short production cycle, the same strain of bacteria, followed by the advantages of solid culture material hair bacteria fast, but generally require equipment. In the absence of fermenter and shaker how to produce liquid bacteria species? I have conducted many tests, now a simple and effective method is described as follows: (a) mother’s preparation using Wang Ziying and other preparation methods, culture Base for the 88% of miscellaneous debris, glucose 10%, 1% protein Dong, yeast 1%, 55% water. After loading lightly compacted tube, and in the middle hit a hole and then sterilized, inoculated, the general culture of mycelium to grow about 12 days. (B) liquid raw material preparation medium for corn flour 5.5% (over 40 mesh sieve), glucose 0.5%, potassium dihydrogen phosphate 0.1%, 0.05% magnesium sulfate, vitamin 0o.01%. Stir well into Krebs bottle or eggplant shape