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目的:研究DXS101位点在中国新疆维吾尔族群体中的遗传结构分布特征。方法:采用PCR扩增,变性聚丙烯酰胺凝胶电泳结合银染显带技术,检测100名(女42,男58)维吾尔族无关个体DXS101位点等位基因及基因型频率分布。结果:在女性样本中,DXS101位点检出9种等位基因和17种基因型;在男性样本中,DXS101位点检出7种等位基因。该位点在女性中的个体识别率为0.8937,多态信息量为0.8072,杂合度为0.8156,在男性中的个体识别率为0.6674。结论:群体遗传多态性指标显示DXS101位点在新疆维吾尔族群体中具有较高多态性,在维吾尔族群体法医学个体识别、亲权鉴定及群体遗传学研究中有重要应用价值。
Objective: To study the distribution of the genetic structure of DXS101 locus in Xinjiang Uygur population. Methods: The frequencies of alleles and genotypes in DXS101 loci of 100 Uighur unrelated individuals (female 42, male 58) were detected by PCR, denaturing polyacrylamide gel electrophoresis and silver staining. Results: In the female samples, nine alleles and 17 genotypes were detected in DXS101 locus. Seven alleles were detected in DXS101 locus in the male samples. The individual identification rate of this locus was 0.8937 in women, the polymorphism information was 0.8072, the heterozygosity was 0.8156, and the individual identification rate was 0.6674 in males. Conclusion: The population genetic polymorphism index shows that the DXS101 locus has high polymorphism in Xinjiang Uighur population, and it has important application value in the forensic identification, paternity test and population genetics of Uighur population.