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４８例正常育龄男子和２４例要求作输精管吻合术者，用免疫珠结合试验（ＩＢＴ）和精子－宫颈粘液接触试验（ＳＣＭＣ）分别检测其近睾端输精管液中的精子（非射出的精子）表面抗精子抗体的情况。结果显示：正常育龄男子非射出的精子上没有抗体存在；而输精管结扎术后非射出的精子上有抗精子抗体存在（ＩｇＧ：７９．４％，ＩｇＡ：３８．２％，ＳＣＭＣ：３５．５％）。这些抗体在输精管结扎术后不到１年就可发现，并保持到结扎术后３年以上。吻合术后１～３个月内，其精液中射出的精子上仍发现抗体存在。结果还提示：输精管结扎术后，不能以非射出的精子上抗体的水平去预测吻合术后射出的精子上抗体的水平；吻合术前血清抗体水平与非射出的精子上抗体水平并不一致。
48 normal fertile men and 24 patients requiring vas deferens anastomosis were tested for spermatozoa (non-ejected spermatozoa) in the spermatic fluid of the proximal testis by immunobead binding assay (IBT) and sperm-cervical mucus contact assay (SCMC) Surface anti-sperm antibodies. The results showed that there was no antibody on non-injected sperms in normal fertile men, whereas anti-sperm antibodies were found on non-injected spermatozoa (IgG: 79.4%, IgA: 38.2%, SCMC: 35.5 %). These antibodies were found less than one year after vasectomy and remained for more than 3 years after ligation. Within 1 to 3 months after anastomosis, the antibodies still exist on the sperm injected from the seminal fluid. The results also suggest that, after vasectomy, the level of non-ejected spermatozoa can not predict the level of sperm antibodies emitted after anastomosis. The level of serum antibody before anastomosis is not consistent with that of non-ejected spermatozoa.