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有关中华猕猴桃蛋白酶(Actinidin,E C 3、4.22.14)催化功能基团的研究表明:CYSH—25、His—161为酶活性部位必需基团,而Lys和Trp残基为酶活力表现所必需。本文中报道应用水溶性碳二亚胺(EDC)修饰动力学方法,进一步探讨羧基
Studies on catalytic functional groups of Actinidin (E C 3,4.22.14) showed that CYSH-25 and His-161 are essential groups in the active site of the enzyme, while Lys and Trp residues are necessary for the activity of the enzyme. This article reports the application of water-soluble carbodiimide (EDC) modification kinetics methods to further explore the carboxyl