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目的 探讨复方木尼孜其颗粒对心肌缺血再灌注损伤(MIRI)大鼠模型的影响及其机制.方法选择96只SD大鼠随机分为8组:正常假手术组(Con组),正常缺血再灌注组(IR组),异常体液载体动物假手术组(AS组),异常体液载体动物心肌缺血再灌注组(AS+IR组),复方木尼孜其颗粒大剂量组(10.12g/kg)、中剂量组(5.06g/kg)、小剂量组(2.53g/kg),阿托伐他汀(AT)组(20mg/kg),每组12只,灌胃干预21天.检测各组心肌组织HSP70、CGRP表达及血清MAD、SOD、IL-6、IL-8含量.结果与IR组比较,AS+IR组、AS组、AT组、小剂量组CGRP表达升高(P＜0.01,P＜0.05);各给药组SOD升高,MDA、IL-6、IL-8降低(P＜0.01).与AS组比较,AS+IR组CGRP表达上调(P＜0.05),大剂量组HSP70表达上调(P＜0.01),各给药组SOD升高(P＜0.01),IL-6降低(P＜0.01),大剂量组及AT组MDA降低(P＜0.01,P＜0.05).与AS+IR组比较,大剂量和中剂量组CGRP表达降低(P＜0.01),各给药组HSP70、SOD升高(P＜0.01),MDA、IL-6及IL-8降低(P＜0.01).结论 复方木尼孜其颗粒可增加MIRI模型大鼠心肌保护性蛋白HSP70的表达,减轻MIRI后心肌的炎症反应,从而达到保护MIRI的疗效.“,”Objective To explore the effect of Munziq Granule on myocardial ischemia reperfusion injury (MIRI) rat and its mechanism.Methods Totally96 rats were divided into 8 groups:the normal Sham (Con) group, normal ischemia-reperfusion (IR) group, model sham (AS) group, ischemia-reperfusion model (AS+IR) group, high dose group (10.12 g/kg) , middle dose group (5.06 g/kg) , low dose group (2.53 g/kg) and atorvastatin intervention (AT) group (20 mg/kg) , 12 rats in each group.All the rats were given intragastric administration for 21 days.The expression of HSP70, CGRP in myocardial tissue were determined by Western Blot, the level of MAD, SOD, IL-6, IL-8 were detected by ELISA.Results Compared with the IR group, CGRP expression level increased in AS+IR group, AS group, AT group, and low dose group (P＜0.01, P＜0.05) ;SOD contents increased, MDA, IL-6 and IL-8 contents decreased in the treated groups (P＜0.01) .Compared with AS group, CGRP expression level increased in the AS+IR group (P＜0.05) ;HSP70 expression level increased in the high dose group (P＜0.01) ;SOD contents increased, IL-6 contents decreased in the treated groups (P＜0.01) ;MDA contents decreased in the high dose group and AT group (P＜0.01, P＜0.05) .Compared with the AS+IR group, CGRP expression level decreased in the high and middle dose groups (P＜0.01) ;SOD contents and HSP70 increased, MDA, IL-6 and IL-8 contents decreased in the treated groups (P＜0.01) .Conclusion Munziq Granule can improve the heart physiological function of model rats with MIRI, and increase the expression of HSP70, while obviously reduce myocarditis, so as to achieve the pro-tective effect of MIRI.