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为了探讨提高小鼠卵细胞质内单精子注入受精率的方法，选取鼠龄１２～１４周的健康昆明白小鼠作为精子和卵子的供体，采用ＩＣＳＩ技术，以受精后二细胞卵裂的形成率为指标，了解不同采卵时间、不同微注射针参数及不同培养液对细胞质内单精子注入的影响。结果表明，ｈＣＧ注射后１８～１９ｈ采卵，用针尖内径为４～５μｍ、斜面角度为３５～４０度的微注射针进行ＩＣＳＩ操作，受精后卵子置ＣＺＢ中培养可获得较多的２细胞胚，卵裂率明显高于其余各组（２８．１０％），有高度显著性差异（Ｐ＜０．０１）。结果提示：恰当的采卵时间、微注射针参数及培养液对ＩＣＳＩ结果有很大影响
In order to explore the method of improving the fertilization rate of single sperm injection in mouse egg cytoplasm, healthy Kunming white mice of 12-14 weeks old were selected as the donor of sperm and egg. The ICSI technique was used to determine the formation of two cell cleavage after fertilization Rate as an indicator to understand the different ovum time, different micro-injection needle parameters and different culture medium on the cytoplasmic single sperm injection. The results showed that eggs were harvested 18 h to 19 h after hCG injection, ICSI was performed by microinjection needle with tip diameter of 4 ~ 5 μm and bevel angle of 35 ~ 40 °. After fertilization, the eggs were cultured in CZB to obtain more 2-cell embryos , Cleavage rate was significantly higher than the other groups (28.10%), there was a highly significant difference (P <0.01). The results suggest that proper oviparous time, microinjection needle parameters and culture medium have a great influence on ICSI results