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目的 探讨维生素E对锰中毒大鼠脑损伤的保护作用.方法 48只Wistar大鼠随机均分为三组.自然组每天腹腔注射生理盐水30 mg/kg;锰中毒组每天腹腔注射氯化锰溶液15 mg/kg;维生素E组在锰中毒组基础上用纯净水稀释维生素E 100 mg/kg灌胃给药,每天1次,共18d.比较三组大鼠Morris水迷宫实验的潜伏期时间和空间探索的平台穿越次数,采用免疫组化尼氏染色观察大鼠脑海马CA3区细胞凋亡情况.结果 水迷宫实验第3、4天,锰中毒组潜伏期时间较自然组和维生素E组延长(P＜0.05).锰中毒组平台探索次数为(1.92±0.78)次,少于自然组的(3.94±1.35)次和维生素E组的(3.85±1.33)次(P＜0.05).锰中毒组凋亡细胞为(59.8±6.4)个,多于维生素E组的(15.8±5.5)个和自然组的(10.5±1.6)个(P＜0.01).结论 维生素E对锰中毒大鼠的脑部损伤有保护作用,提高大鼠的学习记忆能力,减少海马CA3区细胞凋亡.“,”Objective To investigate the protective effect of vitamin E(VE) on the brain injury in rats with manganese poisoning.Methods Forty-eight Wistar rats were equally divided into three groups.Group A was intraperitoneally injected saline 30 mg/kg,group B was intraperitoneally injected manganese chloride 15 mg/kg and group C was intraperitoneally injected with VE 100 mg/kg on the basis of manganese poisoning,once a day for 18 days.The latent time of Morris water maze experiment and time crossing platform were compared among three groups.The cell apoptosis of hippocampus CA3 area was observed by immunohistochemical staining.Results The latent time was longer in group B than that in groups of A and C on the 3rd and 4th day of Morris water maze experiment (P＜0.05).The time crossing platform was less and cell apoptosis was more in group B than those in groups of A and C(P＜0.05 or P＜0.01).Conclusion VE has a protective effect on the brain injury of rats with manganese poisoning,improves the ability of learning and memory and reduces the cell apoptosis of hippocampus CA3 area.